Saturday 16 February 2013

4 Different Types of Column Chromatography

Let us see some of the types of column chromatography. But before knowing that let us check it out definition of chromatography. As we all know that chromatography is a technique applied for the separation of the components of mixture by continuous distribution of the components between two phases, of which one is mobile phase and another one is stationary phase. There are four different types of column chromatography which works on four different mechanisms.

Four different mechanism or types of column chromatography are as follows:

  1. Adsorption chromatography in which the components of mixture are selectively adsorbed on the surface of packing column material i.e. adsorbant.
  2. Partition chromatography in which component is partitioned between the mobile phase and their stationary phase held stationary on inert solid support.
  3. Ion exchange chromatography in which the constituent of sample is selectively retained by exchange resin by replacing ion/s on packing material.
  4. Gel chromatography is the method in which column is packed with a permeable gel which brings out separation by seiving or molecular filtration action.
These are some of the main types of column chromatography which are widely used. There are other types of chromatography such as High performance liquid chromatography , Gas chromatography and much more please check other articles on this blog.

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