Advantages and Disadvantages of Thin Layer Chromatography TLC

 Some advantages and disadvantages of thin layer chromatography are discussed below. So let us check out its advantages and disadvantages to know more about thin layer chromatography.

Some Advantages of Thin Layer Chromatography TLC are:

1. Thin Layer chromatography is a rapid analysis method for complex mixtures.
2. Minimal sample preparation.
3. It has high separation efficiency.
4. Low solvent consumption in thin layer chromatography.
5. It requires small sample volumes.
6. Cost effective method.
7. Instrumentation is simple.
8. Useful for wide range of applications.
9. It is compatible with various detection methods.
10. TLC is easy to perform and interpret. 

Some Disadvantages of Thin Layer Chromatography TLC are:

1. Thin layer chromatography has limited sample capacity.
2. It has low resolution compared to other methods.
3. It has limited reproducibility.
4. TLC has limited sensitivity for some compounds. 
5. Trained person is required to operate.
6. High cost of stationary phase.
7. Limited selectivity for certain analytes.
8. TLC may require multiple development solvents. 

Advantages and Disadvantages of Column Chromatography

 Some advantages and disadvantages of column chromatography are discussed below. So let us find out its advantages and disadvantages to know more about column chromatography.

Advantages of Column Chromatography are:

1. Column chromatography enhances detection of compounds.
2. Column chromatography enables trace analysis.
3. Increases signal to noise ratio
4. It also detects low concentration.
5. Column chromatography improves analytical accuracy.
6. It is best suitable various detection methods.
7. Column chromatography optimizes sensitivity easily.
8. It separates closely related compounds.
9. Enables detection of impurities.

Disadvantages of Column Chromatography are:

1. Column chromatography equipment is expensive. 
2. Costly consumables.
3. The operating cost is high.
4. Column chromatography requires specialized training.
5. It is time consuming process.
6. It is labor intensive technique.
7. Difficult to automate.
8. Requires significate sample for analysis.